Solution Information | help | |
Enzyme: | Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform | |
inhibitor: | BDBM2583 | |
substrate: | Phosphorylase b | |
Solution Type: | Aqueous | |
pH at Preparation: | 7.0 | |
Temp. Prep.: | 30 | |
Comments: | n/a | |
Solute 1: | CaCl2 | |
Concentration: | 0.9 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 2: | MgCl2 | |
Concentration: | 3 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 3: | N-hydroxyethylethylene-diaminetriacetic acid | |
Concentration: | 0.6 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 4: | 2-mercaptoethanol | |
Concentration: | 0.05 % | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
pH Buffer 1: | Tris-HCl | |
Concentration: | 25 mM | |
ΔHion: | n/a | |
Comments: | n/a | |
pH Buffer 2: | Na glycerophosphate | |
Concentration: | 60 mM | |
ΔHion: | n/a | |
Comments: | n/a | |
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